Gene find could lead to healthier food, better biofuel production
January 4, 2011
WEST LAFAYETTE, Ind. - Purdue University scientists have found the last undiscovered gene responsible for the production of the amino acid phenylalanine, a discovery that could lead to processes to control the amino acid to boost plants' nutritional values and produce better biofuel feedstocks.
Natalia Dudareva, a distinguished professor of horticulture, and Hiroshi Maeda, a postdoctoral researcher in Dudareva's laboratory, determined that the gene is one of 10 responsible for phenylalanine production in plants. Understanding how the amino acid is produced could provide a strategy to increase or reduce that production.
Phenylalanine is important for plant protein synthesis and for the production of flower scent, anti-oxidants and lignin, a principal plant cell wall component that helps plants stand upright and acts as a barrier in the production of cellulosic ethanol. It is one of the few essential amino acids that humans and animals cannot synthesize, so it must come from plants.
"In plant tissues where we want to lower lignin content, we may be able to block these pathways," Maeda said. "In cases where you want to increase the amount of phenylalanine, we could do that as well."
Decreasing phenylalanine could lead to a reduction in lignin, which would improve digestibility of cellulosic materials for ethanol production. Increasing phenylalanine could boost the nutritional value of some foods.
Dudareva and Maeda used a co-expression analysis to find the prephenate aminotransferase gene. They monitored the expression activity of nine genes in the research plant Arabidopsis that were known to be involved in phenylalanine production and looked for other genes that became active at the same time.
"This gene had almost identical gene expression patterns as the known phenylalanine-related genes," Maeda said.
The comparable gene in petunias also was identified. Dudareva and Maeda confirmed that its expression patterns matched other genes involved in the formation of phenylalanine and volatile scent compounds in the flower.
To test the find, Dudareva and Maeda used the E. coli bacteria. They overexpressed the protein encoded by newly discovered gene and detected the expected enzyme activity. They also decreased the gene's expression in petunia flowers and witnessed a reduction in phenylalanine production.
"We provided both biochemical and genetic evidence that the gene is indeed involved in phenylalanine biosynthesis," Dudareva said. "It completes the pathway."
Dudareva said she would use the discovery to increase the scent of flowers in order to study the interaction of insects with flowers.
Dudareva and Maeda's findings were published in the early online version of the journal Nature Chemical Biology. The National Science Foundation funded the research.
Writer: Brian Wallheimer, 765-496-2050, email@example.com
Prephenate Aminotransferase Directs Plant Phenylalanine Biosynthesis Via Arogenate
Hiroshi Maeda, Heejin Yoo and Natalia Dudareva
The aromatic amino acids L-phenylalanine and L-tyrosine and their plant-derived natural products are essential in human and plant metabolism and physiology. Here we identified Petunia hybrida and Arabidopsis thaliana genes encoding prephenate aminotransferases (PPA-ATs), thus completing the identification of the genes involved in phenylalanine and tyrosine biosyntheses. Biochemical and genetic characterization of enzymes showed that PPA-AT directs carbon flux from prephenate toward arogenate, making the arogenate pathway predominant in plant phenylalanine biosynthesis.
- Keith Robinson
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June 22, 2016
Groups of high schoolers eagerly lined up Tuesday morning at Purdue University to test how well their handcrafted wind turbines would perform when stacked against the power of four fans. The kids were competing to create a turbine that would generate the most energy as a part of a challenge for the Duke Energy Academy at Purdue. The annual academy, now in its fifth year, brings in U.S. high school students to learn about renewable energy with hopes they'll be inspired to pursue careers in science, technology, engineering and mathematics fields and solve energy challenges. "We want these students to be the leaders of tomorrow," said Pankaj Sharma, managing director of the Purdue Energy Center and Global Sustainability Institute. The academy lasts throughout the week and is hosting 52 students and 27 teachers from mainly Indiana schools, though about 20 percent come from outside states, said Tolu Omotoso, a civil engineering graduate student and coordinator for the academy.Read Full Story