Gregory Knipp

Active Mentor - currently hosting PULSe students for laboratory rotations and recruiting PULSe students into the laboratory; serves on preliminary exam committees

Current Research Interests:

My laboratory is dedicated to the utilization of new and improved models for assessing pharmaceutical performance. The laboratory's research interests include preclinical in vitro and in vivo ADMET screening, physicochemical characterization, early formulation development, and performing pharmacokinetic studies of new candidates and traditional chemical entities. Recently we have focused on the development of a novel, direct contact human blood brain barrier triculture model for screening permeability-linked neuronal response. We have also been collaborating on projects focused on the development of compounds that mitigate viral infections. In addition, I currently serve as the faculty directors of the Purdue Translational Pharmacology (PTP) CTSI Core Facility, where we conduct preclinical pharmacokinetic testing in rodent and the porcine animal models under stress free conditions.

Selected Publications:

J. Wang, X. Yao, S. Toregrosa-Allen, B.D. Elzey, S. Utturkar, N.A. Lanman, V. Bernal-Crespo, M. Behymer, G.T. Knipp, Y. Yun, M.C. Veronesi, A.L. Sinn, K.E. Pollok, R.R. Brutkiewicz, K.S. Nevel, and S. Matosevic. Tumor-responsive, multifunctional CAR-NK cells cooperate with impaired autophagy to infiltrate and target glioblastoma. Proc. Nat. Acad. Sci. USA. 118(45):e2107507118 (2021).

K.E. Lubin and G.T. Knipp. Design of Experiment Based Optimization of an In Vitro Direct Contact Triculture Blood Brain Barrier Model for Permeability Screening. Pharm. Pharmacol. Int. J. 9(4):143-158 (2021).

M. Lavan, X. Wang, R. McCain, A. Jannasch, B. Cooper, S. Hostetler, S. Byrn and G.T. Knipp. Development of a Pediatric Mini-Tablet Formulation for Expedited Preclinical Studies. AAPS PharmSciTech 22:40 (2021)

M. Lavan and G.T. Knipp. Considerations for Determining Direct Versus Indirect Functional Effects of Solubilizing Excipients on Drug Transporters for Enhancing Bioavailability. J. Pharm. Sci. 109(6):1833-1845 (2020).

J. Morningstar, J. Lee, T. Hendry-Hofer, A. Witeof, L.T. Lyle, G. Knipp, C. McCrae, G. Boss, R. Peterson, V.J. Davisson, R. Gerszten, V. Bebarta, S. Mahon, M. Brenner, and A. Nath. Intramuscular Administration of Hexachloroplatinate Reverses Cyanide-Induced Metabolic Derangements and Counteracts Severe Cyanide Poisoning. FASEB BioAdv 1(2):81-92 (2019; Epub October 8, 2018; https://doi.org/10.1096/fba.1024).

N.H. Sulimai, J.C. Ko, Y. Jones-Hall, H.-Y. Weng, M. Deng, G.J. Breur, and G.T. Knipp. Pharmacokinetic and Histopathological Evaluation of 25% Poloxamer as a Slow Release Carrier for Morphine in a Rat Model. Front. Vet. Sci. 5: Article 9 (2018).

C. Kulczar, K.L. Lubin, S. Lefebvre, D.W. Miller, and G.T. Knipp. Development of a Direct Contact Astrocyte-hCMEC/D3 Blood-Brain Barrier Coculture Model. J. Pharm. Pharmacol. 69(12):1684-1696 (2017).

R.P. Gala, C. Popescu, G.T. Knipp*, R.R. McCain, R.V. Ubale, R. Addo, T. Bhowmik, C.D. Kulczar, and M.J. D’Souza*, Physicochemical and Preclinical Evaluation of a Novel Buccal Measles Vaccine. AAPS PharmSciTech [Pediatric Drug Development and Dosage Form Theme Issue] 18(2):1-10 (2017; EPub June 29, 2016; Shared: http://rdcu.be/ndT7).

K.L.R. Brouwer, L.M. Aleksunes, B. Brandys, G.P. Giacoia, G. Knipp, V. Lukacova, B. Meibohm, S.K. Nigam, M. Rieder and S.N. de Wildt; on behalf of the Pediatric Transporter Working Group. Human Ontogeny of Drug Transporters: Review and Recommendations of the Pediatric Transporter Working Group. Clin Pharmacol Ther. 98(3):266-87 (2015).

J.G. Mehtala, C. Kulczar, M. Lavan, G. Knipp* and A. Wei* (*-Co-corresponding authors), Cys34-PEGylated Human Serum Albumin for Drug Binding and Delivery. Bioconjug Chem. 26(5):941-949. (2015). 20.

W.J. Roth, C.B. Kissinger, R.R. McCain, B.R. Cooper, J.N. Marchant-Forde, R.C. Vreeman, S. Hannou, and G.T. Knipp. Assessment of Juvenile Pigs to Serve as Human Pediatric Surrogates for Preclinical Formulation Pharmacokinetic Testing. AAPS J. [Theme Issue: Challenges and Opportunities in Pediatric Drug Development] 15(3):763-774 (2013; EPub: April 18, 2013).

S. Abdel-Rahman, G.L. Amidon, A. Kaul, V. Lukacova, A.A. Vinks, G.T. Knipp, and the Members of the BCS Task Force. Summary of the NICHD-BPCA Pediatric Formulation Initiatives Workshop-Pediatric Biopharmaceutics Classification System (PBCS) Working Group. Clin. Ther. 34(11S):S11-S24 (2012).

R. Kulkarni, N. Yumibe, Z. Wang, X. Zhang, C.C. Tang, K. Ruterbories, A. Cox, R. McCain and G.T. Knipp. Comparative Pharmacokinetic Studies of Immediate and Modified Release Formulations of Glipizide in Pigs and Dogs. J. Pharm. Sci. 101(11):4327-4336 (2012; EPub August 19, 2012).

W.J. Roth, D.J. Lindley, S.M. Carl and G.T. Knipp. The Effects of Intra-Laboratory Modifications to Media Composition and Cell Source on the Expression of Pharmaceutically Relevant Transporters and Metabolizing Genes in the Caco-2 Cell Line. J. Pharm. Sci. 101(10):3962-3978 (2012; EPub: July 11, 2012).